New Trends in Assisted Reproduction Techniques: Cryopreservation, In vitro Fertilization, Intracytoplasmic Sperm Injection and Physiological Intracytoplasmic Sperm Injection
نویسندگان
چکیده
Since that historically the first human birth was registered through in vitro fertilization in 1978, the improvement and creation of new assisted reproduction techniques such as intracytoplasmic sperm injection and physiological intracytoplasmic sperm injection have become of great importance nowadays. Louise Brown was the first baby conceived through in vitro fertilization in England. Since then, several worldwide studies have reported human and domestic animals outcomes. This fact has solved human fertility problems creating new ways of conceiving. Before the success with in vitro fertilization, other assisted reproduction techniques such as cryopreservation were developed. Spermatozoa were the first mammalian cells to be cryopreserved. These events revolutionized all the research in the field of reproductive biology. However, in terms of cryopreservation, intense work needs to be intended since low recovery rates are still reported. The improvement of vitrification-warming procedures is highly important in order to increase embryo development and outcomes. In domestic species, porcine oocytes are the most sensitive cells during vitrification. The future of vitrification success is promising; therefore cryobiologists have a commitment in the field of assisted reproduction. The aim of this review was the acknowledgement of the current status of assisted reproduction techniques, its application, progress and future prospects.
منابع مشابه
The Outcomes of Intracytoplasmic Sperm Injection and Laser Assisted Hatching in Women Undergoing In Vitro Fertilization Are Affected by The Cause of Infertility
Background We sought to determine the association between factors that affected clinical pregnancy and live birth rates in patients who underwent in vitro fertilization (IVF) and received intracytoplasmic sperm injection (ICSI) and/or laser assisted hatching (LAH), or neither. MaterialsAndMethods In this retrospective cohort study, the records of women who underwent IVF with or without ICSI and...
متن کاملCryopreservation of testicular and epididymal sperm: techniques and clinical outcomes of assisted conception
The introduction of the technique of intracytoplasmic sperm injection to achieve fertilization, especially using surgically retrieved testicular or epididymal sperm from men with obstructive or non-obstructive azoospermia, has revolutionized the field of assisted reproduction. The techniques for the retrieval of spermatozoa vary from relatively simple percutaneous sperm aspiration to open excis...
متن کاملCryopreservation of sperm: indications, methods and results.
PURPOSE We review the history of the cryopreservation of human sperm. The current methods of cryopreservation and indications are highlighted, and the success rates of the various uses of cryopreserved sperm are reviewed. Potential adverse sequelae of human cryopreservation are also discussed. MATERIALS AND METHODS Indications for cryopreservation include several applications, namely homologo...
متن کاملRelationship between Endometrial Thickness and In Vitro Fertilization-Intracytoplasmic Sperm Injection Outcome
متن کامل
Infertility in the Modern World: Present and Future Prospects
17 Introduction 18 Ovarian stimulation 20 In vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) 23 Immature sperm conception 25 Egg donation and surrogacy 28 Oocyte maturation 28 Embryo culture 30 Assisted hatching 31 Cryopreservation 32 Preimplantation genetic diagnosis (PGD) 34 Cytoplasmic donation and mitochondrial DNA (mDNA) transfer 37 Fertility preservation 39 Conclusion 41
متن کامل